WebMature tRNAs were purified by denaturing 10% TBE-Urea PAGE followed by ligation with the I4 3’ adapter using T4 RNA ligase 2, truncated KQ (New England Biolabs, M0373L). For primer-dependent reverse transcription, adapter-ligated tRNAs were incubated with TGIRT (InGex, TGIRT50) in low-salt buffer (50 mM Tris-HCl, pH 8.0, 75 mM KCl, 3 mM … WebNew England Biolabs Certificate of Analysis B0202S / Lot: 10182377 Page 1 of 2 ... substrate RNA remains intact as determined by fluorescent detection. Pass pH (buffers/solutions) The pH of 10X T4 DNA Ligase Reaction Buffer is between pH 7.4 and 7.6 at 25°C. Pass. 240 County Road Ipswich, MA 01938-2723 Tel 978-927-5054 Fax 978 …
T4 RNA Ligase 2, truncated NEB
WebA 20 µl reaction in 1X T4 DNA Ligase Reaction Buffer containing 2 µg of Lambda DNA-HindIII Digest and a minimum of 4000 units of T4 DNA Ligase incubated for 16 hours at 37°C results in >95% ligation of the DNA fragments as determined by agarose gel electrophoresis. Of these ligated fragments, >95% can be recut with HindIII. Pass Web2. Add 1.0 µl of Quick T4 DNA Ligase. Vortex briefly to mix, followed by a quick spin. 3. Incubate for 10 minutes at 25°C. ... labeled RNA transcript for 16 hours at 37°C results in no detectable RNase activity as determined ... Version 2.1 9/12 USA New England Biolabs, Inc. 240 County Road Ipswich, MA 01938-2723 Telephone: (978) 927-5054 ... michelle craske publications
manual NEBNext DNA Library Prep Master Mix Set for 454 …
WebMar 27, 2024 · Then, a 2 μl mixture containing 0.01 U Bst 2.0 DNA polymerase, indicated units of SplintR/T3 DNA ligase/T4 DNA ligase/ E. coli DNA ligase/Taq DNA ligase/Electrol ligase/T7 DNA ligase (T7Dnl) (NEB), 10 nM ATP, and 5 μM dNTP were added to hybridization products and incubated at 16°C/25°C for 1 h, or 37°C/50°C/60°C for 30 min … WebNEBioCalculator Ligation Calculator This tool will calculate the mass of insert required at several molar insert:vector ratios in the range needed for typical ligation reactions. Ligation Tutorials Insert DNA length Vector DNA length Vector DNA mass Required insert DNA mass --- (1:1) --- (2:1) --- (3:1) --- (5:1) --- (7:1) Formula WebOur T4 RNA Ligase 2 Truncated demonstrates equivalent or superior volume/volume performance when compared to analogous T4 RNA Ligase 2 Truncated products on the market. The full length and truncated T4 RNA Ligase 2 are supplied in 10 mM Tris-HCl, 100 mM NaCl, 0.1 mM DTT, 0.1 mM EDTA and 50% glycerol; pH 7.5 at 25°C. michelle crawfordville fl facebook