Web31. júl 2015 · pENTR D TOPO Gateway Cloning Most recent answer 29th Jun, 2016 Renan Pioli MCassab Here are some tips to get colonies: . high concentration of pcr products (up to 60 ng/uL) . gel purification .... Web24. okt 2013 · C) Topo cloning vector 1uL (fixed volume) D) sterile water to 6uL total. The manual reads "For optimal results, be sure to use a 0.5:1–2:1 molar ratio of PCR product:TOPO® vector in your TOPO® Cloning reaction." It also reads that use of "5–10 ng of a 2-kb PCR product in a TOPO® Cloning reaction generally results in a suitable number …

Vector Backbone pENTR-D-TOPO - genomics-online.com

WebPentrtm D Topo Entry Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, … WebThe pENTR™/D-TOPO™ vector (Figure 1) includes M13 and T7 primer sequencing sites and attL recombination sites flanking the PCR product insertion site. This allows clones to be … cda eastland near me

pENTRTM Directional TOPO® Cloning Kits - 豆丁网

Webuser guide For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. pENTR™ Directional TOPO® Cloning Kits Five-minute, directional TOPO® … WebpENTR/SD/D-TOPO vectorincludes T7 gene 10 translational enhancer and ribosome binding site (RBS) for optimal expression of native protein after recombination with prokaryotic Gateway destination vectors Can also use vector for expression in other hosts systems by recombination with different destination vectors WebpENTR™⁄D-TOPO™ Cloning Kit contains pENTR⁄D-TOPO™ vector, dNTPs, salt solution, sterile water, universal M13 sequencing primers, OneShot™ TOP10 Chemically E. coli, S.O.C. Medium, and a pUC19 control plasmid. Store Competent E. coli at -80°C. Store all other components at -20°C. Vector: pENTR cda don\\u0027t worry darling